ABSTRACT
Infectious diarrhoea in neonates of animals is one of the most common and economically important conditions encountered in the livestock industry. Faecal samples (n=210) from diarrhoeic neonatal goat-kids of different livestock sheds of ICAR-CIRG, Makhdoom, Mathura (U.P.), were aseptically collected, and immediately processed for isolation of bacterial pathogens and parasitic evaluation. A total of 178 isolates of E. coli from 210 samples were identified on the basis of cultural, morphological, biochemical and molecular characteristics. Out of 178 E. coli isolates, 3.93 % (7/178) isolates were identified as STEC by PCR amplification of stx-1 and stx-2 gene. A total of 64 isolates of E. coli were sent to National Salmonella and Escherichia Centre, Central Research Institute, Kasauli for the serotyping. The common serogroups of E. coli responsible for neonatal diarrhoea in goat-kids were identified as O36, O26, O59, O29, O43, O91, O82, O9 and O171, out of which, 46.15% were O36, O26 and O59. Cryptosporidium spp. infection was detected in 46 samples out of 148 faecal samples by ZN staining and nested PCR.Based on cultural, morphological, biochemical and molecular characteristics,16 isolates of Salmonella spp. and 5 of Klebsiella spp. were identified from 210 fecal samples. The present study concluded that E. coli followed by Cryptosporidium spp. and Salmonella spp. were the prevalent infectious agents associated with neonatal diarrhoea in goat-kids
ABSTRACT
Faecal samples (n=300) from diarrhoeic neonatal goat-kids of different livestock sheds of ICAR-CIRG, Makhdoom, and field goat-kids of Mathura, UP were aseptically collected, and used for E. coli isolation. On the basis of cultural, morphological, biochemical and molecular characteristics, a total of 193 E. coli isolates were identified from 300 fecal samples. Out of 140 E. coli isolates, only 90 isolates could be serotyped at National Salmonella and Escherichia Centre, Central Research Institute, Kasauli, and the most common serogroups responsible for neonatal diarrhoea were found as O88 (n=11), O22 (n=10), O11 (n=8) and O83 (n=7). Congo red dye agar test was done to determine invasiveness of the isolates, and 77.20% (149/193)E. coli isolates showed Congo red binding activity. Identification of shiga toxin producing E. coli (STEC) was done by PCR amplification of its stx-1 gene, and 5.69% (11/193) isolates were identified as STEC. Pathotype specific primers were used to amplify bundle forming pilus (bfpA) gene of enteropathogenic E. coli (EPEC), and 35.23% (68/193) isolates were identified as EPEC. A multiplex PCR was performed to detect labile toxin producing enterotoxigenic E. coli (ETEC-lt), stable toxin producing enterotoxigenic E. coli (ETEC-st) and enteroinvasive E. coli (EIEC), and 24.35% (47/193), 2.59% (5/193) and 2.07% (4/193) isolates were determined as ETEC-st, ETEC-lt and EIEC, respectively. EPEC and ETEC-st were found as the most prevalent pathotypes associated with neonatal diarrhoea in goat-kids whereas; O88 and O22 were observed as the most common serogroups in causing diarrhoea in the neonatal goat-kids.